服務(wù)熱線
021-54479081
021-54461587
產(chǎn)品展示PRODUCTS
本產(chǎn)品僅用于科研,不用于臨床
小鼠肺部活化調(diào)節(jié)趨化因子酶免試劑盒,(PARC/CCL18)ELISA檢測試劑盒 ,英文名:PARC/CCL18 ELISA Kit ,英文縮寫:PARC/CCL18 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠肺部活化調(diào)節(jié)趨化因子ELISA檢測試劑盒,PARC/CCL18 檢測試劑盒, PARC/CCL18 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠肺部活化調(diào)節(jié)趨化因子酶免試劑盒,(PARC/CCL18)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結(jié)合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結(jié)果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標本中與反應(yīng)無關(guān)的成分、游離的酶標物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結(jié)果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標儀具有良好的重復性。
Misato同源物1(MSTO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Misato Homolog 1 (MSTO1)48T/96TY13985Mus musculus (Mouse)
蛋白酶3(PR3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Proteinase 3 (PR3)48T/96TY13986Mus musculus (Mouse)
輕肽神經(jīng)絲蛋白(NEFL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neurofilament, Light Polypeptide (NEFL)48T/96TY13987Mus musculus (Mouse)
DNA激活蛋白激酶催化亞基肽(PRKDC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC)48T/96TY13988Mus musculus (Mouse)
?;人崴饷福ˋOAH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Acyloxyacyl Hydrolase (AOAH)48T/96TY13989Homo sapiens (Human)
龍蝦肽酶樣金屬內(nèi)肽酶(ASTL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Astacin Like Metallo Endopeptidase (ASTL)48T/96TY13990Homo sapiens (Human)
酪蛋白β(CSN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Casein Beta (CSN2)48T/96TY13991Bos taurus; Bovine (Cattle)
半胱氨酸豐富分泌蛋白3(CRISP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cysteine Rich Secretory Protein 3 (CRISP3)48T/96TY13992Homo sapiens (Human)
2',5'-寡腺苷酸合成酶2(OAS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for 2',5'-Oligoadenylate Synthetase 2 (OAS2)48T/96TY13993Homo sapiens (Human)
2',5'-寡腺苷酸合成酶3(OAS3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for 2',5'-Oligoadenylate Synthetase 3 (OAS3)48T/96TY13994Homo sapiens (Human)
降鈣素原(PCT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Procalcitonin (PCT)48T/96TY13995Rattus norvegicus (Rat)
白介素10受體α(IL10Rα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 10 Receptor Alpha (IL10Ra)48T/96TY13996Homo sapiens (Human)
死亡關(guān)聯(lián)蛋白激酶1(DAPK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Death Associated Protein Kinase 1 (DAPK1)48T/96TY13997Mus musculus (Mouse)
成纖維細胞激活蛋白α(FAPα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Activation Protein Alpha (FAPa)48T/96TY13998Homo sapiens (Human)
二酰甘油-O-?;D(zhuǎn)移酶同源物1(DGAT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1)48T/96TY13999Rattus norvegicus (Rat)
大腸桿菌宿主殘留蛋白(ECP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Escherichia coli Protein (ECP)48T/96TY14000General
纖維蛋白原相關(guān)蛋白1(FGL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrinogen Like Protein 1 (FGL1)48T/96TY14001Mus musculus (Mouse)
剪切修復交叉互補修復缺陷偶聯(lián)因子1(ERCC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1)48T/96TY14002Homo sapiens (Human)
死亡關(guān)聯(lián)蛋白激酶3(DAPK3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Death Associated Protein Kinase 3 (DAPK3)48T/96TY14003Mus musculus (Mouse)
核有絲分裂器蛋白1(NUMA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nuclear Mitotic Apparatus Protein 1 (NUMA1)48T/96TY14004Homo sapiens (Human)
S100鈣結(jié)合蛋白A2(S100A2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for S100 Calcium Binding Protein A2 (S100A2)48T/96TY14005Homo sapiens (Human)
血管緊張素1-7(Ang1-7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiotensin 1-7 (Ang1-7)48T/96TY14006Mus musculus (Mouse)
驅(qū)動蛋白家族成員14(KIF14)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kinesin Family, Member 14 (KIF14)48T/96TY14007Homo sapiens (Human)
V-Myc骨髓細胞瘤病毒癌基因同源物(MYC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC)48T/96TY14008Mus musculus (Mouse)
分泌粒蛋白Ⅲ(SCG3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secretogranin III (SCG3)48T/96TY14009Homo sapiens (Human)
白細胞關(guān)聯(lián)免疫球蛋白樣受體1(LAIR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leukocyte Associated Immunoglobulin Like Receptor 1 (LAIR1)48T/96TY14010Homo sapiens (Human)
鳥氨酸脫羧酶(ODC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ornithine Decarboxylase (ODC)48T/96TY14011Mus musculus (Mouse)
表皮轉(zhuǎn)谷氨酰胺酶(TGM3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transglutaminase 3, Epidermal (TGM3)48T/96TY14012Mus musculus (Mouse)
角質(zhì)轉(zhuǎn)谷氨酰胺酶(TGM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transglutaminase 1, Keratinocyte (TGM1)48T/96TY14013Mus musculus (Mouse)
干擾素α(IFNα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Alpha (IFNa)48T/96TY14014Ovis aries; Ovine (Sheep)
凋亡蛋白酶激活因子1(APAF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1)48T/96TY14015Mus musculus (Mouse)
凋亡蛋白酶激活因子1(APAF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1)48T/96TY14016Rattus norvegicus (Rat)
白介素31(IL31)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 31 (IL31)48T/96TY14017Homo sapiens (Human)
多效生長因子(PTN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pleiotrophin (PTN)48T/96TY14018Bos taurus; Bovine (Cattle)
多效生長因子(PTN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pleiotrophin (PTN)48T/96TY14019Mus musculus (Mouse)
多效生長因子(PTN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pleiotrophin (PTN)48T/96TY14020Rattus norvegicus (Rat)
絲切蛋白1(CFL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cofilin 1, Non Muscle (CFL1)48T/96TY14021Mus musculus (Mouse)
絲切蛋白1(CFL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cofilin 1, Non Muscle (CFL1)48T/96TY14022Rattus norvegicus (Rat)
淋巴毒素β(LTβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lymphotoxin Beta (LTb)48T/96TY14023Mus musculus (Mouse)
淋巴毒素β(LTβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lymphotoxin Beta (LTb)48T/96TY14024Rattus norvegicus (Rat)
溶酶體關(guān)聯(lián)膜蛋白1(LAMP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lysosomal Associated Membrane Protein 1 (LAMP1)48T/96TY14025Homo sapiens (Human)
CD200受體2(CD200R2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for CD200 Receptor 2 (CD200R2)48T/96TY14026Homo sapiens (Human)
膽綠素還原酶B(BLVRB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Biliverdin Reductase B (BLVRB)48T/96TY14027Homo sapiens (Human)
膽綠素還原酶A(BLVRA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Biliverdin Reductase A (BLVRA)48T/96TY14028Homo sapiens (Human)
粒細胞趨化蛋白2(GCP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Granulocyte Chemotactic Protein 2 (GCP2)48T/96TY14029Mus musculus (Mouse)
二級淋巴組織趨化因子(SLC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secondary Lymphoid Tissue Chemokine (SLC)48T/96TY14030Mus musculus (Mouse)
B-淋巴細胞趨化因子1(BLC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for B-Lymphocyte Chemoattractant 1 (BLC1)48T/96TY14031Mus musculus (Mouse)
B-淋巴細胞趨化因子1(BLC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for B-Lymphocyte Chemoattractant 1 (BLC1)48T/96TY14032Rattus norvegicus (Rat)
胸腎表達趨化因子(BRAK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Breast And Kidney Expressed Chemokine (BRAK)48T/96TY14033Rattus norvegicus (Rat)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。