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小鼠視黃醇結合蛋白4酶免試劑盒,(RBP-4)ELISA檢測試劑盒 ,英文名:RBP-4 ELISA Kit ,英文縮寫:RBP-4 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠視黃醇結合蛋白4ELISA檢測試劑盒,RBP-4 檢測試劑盒, RBP-4 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠視黃醇結合蛋白4酶免試劑盒,(RBP-4)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發(fā)生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質。可用洗板機洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
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生長分化因子2(GDF2)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Growth Differentiation Factor 2 (GDF2)96TY17500Mus musculus (Mouse)
生長分化因子2(GDF2)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Growth Differentiation Factor 2 (GDF2)96TY17501Rattus norvegicus (Rat)
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干擾素γ誘導單核因子(MIγ)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Monokine Induced By Interferon Gamma (MIg)96TY17507Homo sapiens (Human)
干擾素γ誘導單核因子(MIγ)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Monokine Induced By Interferon Gamma (MIg)96TY17508Mus musculus (Mouse)
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表皮調節(jié)素(EREG)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Epiregulin (EREG)96TY17510Mus musculus (Mouse)
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白介素4誘導蛋白1(IL4I1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interleukin 4 Induced Protein 1 (IL4I1)96TY17516Homo sapiens (Human)
白介素4誘導蛋白1(IL4I1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interleukin 4 Induced Protein 1 (IL4I1)96TY17517Mus musculus (Mouse)
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干擾素誘導跨膜蛋白2(IFITM2)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interferon Induced Transmembrane Protein 2 (IFITM2)96TY17519Mus musculus (Mouse)
干擾素誘導跨膜蛋白2(IFITM2)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interferon Induced Transmembrane Protein 2 (IFITM2)96TY17520Rattus norvegicus (Rat)
干擾素誘導跨膜蛋白3(IFITM3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interferon Induced Transmembrane Protein 3 (IFITM3)96TY17521Homo sapiens (Human)
干擾素誘導跨膜蛋白3(IFITM3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interferon Induced Transmembrane Protein 3 (IFITM3)96TY17522Mus musculus (Mouse)
干擾素誘導跨膜蛋白3(IFITM3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interferon Induced Transmembrane Protein 3 (IFITM3)96TY17523Rattus norvegicus (Rat)
含跨膜Emp24蛋白運輸域蛋白1(TMED1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Transmembrane Emp24 Protein Transport Domain Containing Protein 1 (TMED1)96TY17524Homo sapiens (Human)
含跨膜Emp24蛋白運輸域蛋白1(TMED1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Transmembrane Emp24 Protein Transport Domain Containing Protein 1 (TMED1)96TY17525Mus musculus (Mouse)
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三角形四肽重復干擾素誘導蛋白1(IFIT1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interferon Induced Protein With Tetratricopeptide Repeats 1 (IFIT1)96TY17527Homo sapiens (Human)
三角形四肽重復干擾素誘導蛋白1(IFIT1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interferon Induced Protein With Tetratricopeptide Repeats 1 (IFIT1)96TY17528Mus musculus (Mouse)
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胰島素樣生長因子2-mRNA結合蛋白1(IGF2BP1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Insulin Like Growth Factor 2 mRNA Binding Protein 1 (IGF2BP1)96TY17533Homo sapiens (Human)
胰島素樣生長因子2-mRNA結合蛋白1(IGF2BP1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Insulin Like Growth Factor 2 mRNA Binding Protein 1 (IGF2BP1)96TY17534Mus musculus (Mouse)
胰島素樣生長因子2-mRNA結合蛋白1(IGF2BP1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Insulin Like Growth Factor 2 mRNA Binding Protein 1 (IGF2BP1)96TY17535Rattus norvegicus (Rat)
胰島素樣生長因子2-mRNA結合蛋白2(IGF2BP2)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Insulin Like Growth Factor 2 mRNA Binding Protein 2 (IGF2BP2)96TY17536Homo sapiens (Human)
胰島素樣生長因子2-mRNA結合蛋白2(IGF2BP2)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Insulin Like Growth Factor 2 mRNA Binding Protein 2 (IGF2BP2)96TY17537Mus musculus (Mouse)
胰島素樣生長因子2-mRNA結合蛋白2(IGF2BP2)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Insulin Like Growth Factor 2 mRNA Binding Protein 2 (IGF2BP2)96TY17538Rattus norvegicus (Rat)
胰島素樣生長因子2-mRNA結合蛋白3(IGF2BP3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3)96TY17539Homo sapiens (Human)
胰島素樣生長因子2-mRNA結合蛋白3(IGF2BP3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3)96TY17540Mus musculus (Mouse)
胰島素樣生長因子2-mRNA結合蛋白3(IGF2BP3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3)96TY17541Rattus norvegicus (Rat)
白介素1受體樣蛋白2(IL1RL2)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interleukin 1 Receptor Like Protein 2 (IL1RL2)96TY17542Homo sapiens (Human)
白介素1受體樣蛋白2(IL1RL2)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interleukin 1 Receptor Like Protein 2 (IL1RL2)96TY17543Mus musculus (Mouse)
白介素1受體樣蛋白2(IL1RL2)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interleukin 1 Receptor Like Protein 2 (IL1RL2)96TY17544Rattus norvegicus (Rat)
Aristaless樣同源框蛋白4(ALX4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Aristaless Like Homeobox Protein 4 (ALX4)96TY17545Homo sapiens (Human)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。